Limited GADD45α expression and function in IL-1β toxicity towards insulin-producing cells.

نویسندگان

  • Lukasz Skalniak
  • Ewa Gurgul-Convey
  • Katarzyna Okreglicka
  • Anna Skalniak
  • Jolanta Jura
چکیده

Growth arrest and DNA damage-inducible (GADD) 45 proteins are regulators of cell death and survival. The proinflammatory cytokine IL-1β strongly increases the level of the transcript encoding GADD45α in rat insulin-producing INS-1E cells. The activation of Gadd45α gene is clearly dependent on JNK and NF-κB activation and the synthesis of the secondary mediator nitric oxide (NO). Interestingly, the observed twelve-fold increase in the GADD45α-coding transcript level is not followed by increased expression of GADD45α at the protein level. An analysis of IL-1β toxicity in INS-1E cells overexpressing GADD45α revealed no correlation between the GADD45α protein level and the sensitivity to IL-1β toxicity. These findings suggest that the potential engagement of GADD45α in IL-1β toxicity towards beta cells is limited to the effects induced by the basal expression level of this protein.

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عنوان ژورنال:
  • Acta biochimica Polonica

دوره 60 4  شماره 

صفحات  -

تاریخ انتشار 2013